Hotspot

tRNA-Ser-tRNA-Gly

LSC · 8248-9208 · 961 bp

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Pi 0.00919

MarkerSeek score 60.40

Primer available yes

Label tRNA-Ser-tRNA-Gly

Feature Metadata

label_name: tRNA-Ser-tRNA-Gly
feature_type: igs
region: LSC
length_bp: 961
variable_sites: 37
pi: 0.00919
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Ser-tRNA-Gly_p1	GAAGTGATCAGAAGGAGCGG	AACCGAAAGACCCCTTAACT	55.0	45.0	58.1	56.4	1132-1169 bp	1.0	71.3
tRNA-Ser-tRNA-Gly_p2	AATCTGCCGCTTTAGTCCAC	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	1061-1098 bp	1.0	70.1
tRNA-Ser-tRNA-Gly_p3	CAATCTGCCGCTTTAGTCCA	AACCGAAAGACCCCTTAACT	50.0	45.0	58.3	56.4	1062-1099 bp	1.0	70.1
tRNA-Ser-tRNA-Gly_p4	GAAGTGATCAGAAGGAGCGG	AAACCGAAAGACCCCTTAACT	55.0	42.9	58.1	57.1	1133-1170 bp	1.0	69.4
tRNA-Ser-tRNA-Gly_p5	ATCAGAAGGAGCGGAGAGAG	AACCGAAAGACCCCTTAACT	55.0	45.0	58.3	56.4	1126-1163 bp	1.0	69.4