Hotspot

rps16-tRNA-Gln

LSC · 5729-6957 · 1229 bp

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Pi 0.0086

MarkerSeek score 70.50

Primer available yes

Label rps16-tRNA-Gln

Feature Metadata

label_name: rps16-tRNA-Gln
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 48
pi: 0.0086
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-tRNA-Gln_p1	AAGTCGCACGTTGCTTTCTA	CAAGTGGTAAGGCAATGGGT	45.0	50.0	58.1	58.1	1292-1350 bp	1.0	85.1
rps16-tRNA-Gln_p2	TCATGTCCTTCAAGTCGCAC	CAAGTGGTAAGGCAATGGGT	50.0	50.0	58.2	58.1	1303-1361 bp	1.0	84.6
rps16-tRNA-Gln_p3	AAGTCGCACGTTGCTTTCTA	GGGTTTTGGTCCCGTTACTC	45.0	55.0	58.1	58.5	1276-1334 bp	1.0	82.0
rps16-tRNA-Gln_p4	TCATGTCCTTCAAGTCGCAC	GGGTTTTGGTCCCGTTACTC	50.0	55.0	58.2	58.5	1287-1345 bp	1.0	81.5
rps16-tRNA-Gln_p5	AAGTCGCACGTTGCTTTCTA	TAAATCGAAATGGGGCGTGG	45.0	50.0	58.1	58.6	1313-1371 bp	1.0	80.8