Hotspot

ycf1

IRb · 109719-112007 · 2289 bp

Back to Libanotis

Pi 0.00716

MarkerSeek score 45.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 2289
variable_sites: 42
pi: 0.00716
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 45.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CCCGGAACTTCTTCGTAGTG	GGGAATAAGAGGATTGGCCG	55.0	55.0	58.0	58.1	2724 bp	0.076923	42.2
ycf1_p2	CCGGAACTTCTTCGTAGTGG	GGGAATAAGAGGATTGGCCG	55.0	55.0	58.0	58.1	2723 bp	0.076923	42.2
ycf1_p3	ATCTCCCCAGGTAGGATTCG	GGGAATAAGAGGATTGGCCG	55.0	55.0	58.0	58.1	2880 bp	0.076923	42.1
ycf1_p4	TCAAAGCGAGCCCTTTTCTT	GGGAATAAGAGGATTGGCCG	45.0	55.0	58.0	58.1	2933 bp	0.076923	42.1
ycf1_p5	AACCCATGACCAATACGAGC	GGGAATAAGAGGATTGGCCG	50.0	55.0	58.0	58.1	2764 bp	0.076923	42.0