Hotspot

psbA

LSC · 334-1395 · 1062 bp

Back to Libanotis

Pi 0.00237

MarkerSeek score 37.60

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 11
pi: 0.00237
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	AAATTCGTGTGCTTGGGAGT	55.0	45.0	58.1	58.0	1319-1359 bp	1.0	83.1
psbA_p2	CAATCCACTGCCTTGATCCA	AAATTCGTGTGCTTGGGAGT	50.0	45.0	57.9	58.0	1333-1373 bp	1.0	83.1
psbA_p3	AATCCACTGCCTTGATCCAC	AAATTCGTGTGCTTGGGAGT	50.0	45.0	57.9	58.0	1332-1372 bp	1.0	83.0
psbA_p4	ACAATCCACTGCCTTGATCC	AAATTCGTGTGCTTGGGAGT	50.0	45.0	57.9	58.0	1334-1374 bp	1.0	83.0
psbA_p5	GATCCACTTGGCTACATCCG	TGCTTGGGAGTCCCTGATAA	55.0	50.0	58.1	58.0	1310-1350 bp	1.0	83.0