Hotspot

ycf2-trnL-CAA

IRb · 93217-94469 · 1253 bp

Back to Libanotis

Pi 0.033
MarkerSeek score 80.90
Primer available yes
Label ycf2-trnL-CAA

Feature Metadata

label_name
ycf2-trnL-CAA
feature_type
igs
region
IRb
length_bp
1253
variable_sites
114
pi
0.033
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2-trnL-CAA_p1 ATGGCTTTTCCCGGATGAAA AAATGGTAGACACGCGAGAC 45.0 50.0 57.8 58.0 500-1512 bp 1.0 89.5
ycf2-trnL-CAA_p2 TGGCTTTTCCCGGATGAAAT AAATGGTAGACACGCGAGAC 45.0 50.0 57.8 58.0 499-1511 bp 1.0 89.5
ycf2-trnL-CAA_p3 ATGGCTTTTCCCGGATGAAA TGAAATGGTAGACACGCGAG 45.0 50.0 57.8 58.0 502-1514 bp 1.0 89.5
ycf2-trnL-CAA_p4 TGGCTTTTCCCGGATGAAAT TGAAATGGTAGACACGCGAG 45.0 50.0 57.8 58.0 501-1513 bp 1.0 89.5
ycf2-trnL-CAA_p5 AAGATGGCTTTTCCCGGATG AAATGGTAGACACGCGAGAC 50.0 50.0 58.2 58.0 504-1515 bp 0.923077 86.0