Hotspot

psbE-petL

LSC · 65368-66372 · 1005 bp

Back to Libanotis

Pi 0.00476

MarkerSeek score 69.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1005
variable_sites: 22
pi: 0.00476
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	1092-1177 bp	1.0	85.2
psbE-petL_p2	ACTGACGAATAACCAACCCG	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.9	58.0	1105-1190 bp	1.0	84.9
psbE-petL_p3	CAACCCGCAATGAATAGGGA	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	1095-1180 bp	1.0	84.6
psbE-petL_p4	AGAACGTTCTCCTGTGCTTC	GGTTACCGTTAAAGCCGCTA	50.0	50.0	57.8	58.0	1015-1100 bp	1.0	84.5
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGAGGTTACCGTTAAAGCCG	50.0	50.0	57.9	57.9	1108-1193 bp	1.0	84.3