Hotspot

ndhC-trnV-UAC

LSC · 51587-52671 · 1085 bp

Back to Libanotis

Pi 0.0103

MarkerSeek score 68.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1085
variable_sites: 43
pi: 0.0103
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCAGGCCCTTTGTTAATCGG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.9	58.1	1204-1316 bp	1.0	84.4
ndhC-trnV-UAC_p2	TAATCGGGGCTAAAACTCCG	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.4	58.1	1191-1303 bp	1.0	80.6
ndhC-trnV-UAC_p3	TCAGGCCCTTTGTTAATCGG	GTTCGAGTCCGTATAGCCCT	50.0	55.0	57.9	58.7	1189-1301 bp	1.0	79.9
ndhC-trnV-UAC_p4	CCCTTTGTTAATCGGGGCTA	CCGAGAAAGTCTACGGTTCG	50.0	55.0	57.3	58.1	1199-1311 bp	1.0	79.7
ndhC-trnV-UAC_p5	GGGGCTAAAACTCCGGAAAC	CCGAGAAAGTCTACGGTTCG	55.0	55.0	58.8	58.1	1186-1298 bp	1.0	78.9