Hotspot

trnT-GGU-psbD

LSC · 32320-33763 · 1444 bp

Back to Libanotis

Pi 0.00722
MarkerSeek score 69.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1444
variable_sites
44
pi
0.00722
unique_haplotype_count
13
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AAGGGCCCTTTTAACTCAGC AGACCGGACCATCCTACAAA 50.0 50.0 58.1 58.1 1619-1656 bp 1.0 83.3
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA AGACCGGACCATCCTACAAA 50.0 50.0 57.9 58.1 1615-1652 bp 1.0 83.1
trnT-GGU-psbD_p3 AAGGGCCCTTTTAACTCAGC GAGCAATAGACCGGACCATC 50.0 55.0 58.1 57.8 1626-1663 bp 1.0 82.5
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA GAGCAATAGACCGGACCATC 50.0 55.0 57.9 57.8 1622-1659 bp 1.0 82.3
trnT-GGU-psbD_p5 AAGGGCCCTTTTAACTCAGC AGAGCAATAGACCGGACCAT 50.0 50.0 58.1 58.2 1627-1664 bp 1.0 82.1