Hotspot

trnE-UUC-trnT-GGU

LSC · 31498-32247 · 750 bp

Back to Libanotis

Pi 0.0106

MarkerSeek score 71.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 750
variable_sites: 28
pi: 0.0106
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CGTTGCCTCCTTGAAAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.8	58.0	419-884 bp	1.0	85.5
trnE-UUC-trnT-GGU_p2	GTGTAGTACCCTACCCCCAG	GAACCGATGACTTACGCCTT	60.0	50.0	58.2	58.0	454-919 bp	1.0	85.0
trnE-UUC-trnT-GGU_p3	CGTTGCCTCCTTGAAAGAGA	TACCGCTGAGTTAAAAGGGC	50.0	50.0	57.8	57.9	384-849 bp	1.0	84.8
trnE-UUC-trnT-GGU_p4	CGTTGCCTCCTTGAAAGAGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.8	58.2	416-881 bp	1.0	84.4
trnE-UUC-trnT-GGU_p5	GTGTAGTACCCTACCCCCAG	TACCGCTGAGTTAAAAGGGC	60.0	50.0	58.2	57.9	419-884 bp	1.0	84.3