Hotspot

psbM-trnD-GUC

LSC · 30384-31071 · 688 bp

Back to Libanotis

Pi 0.00775

MarkerSeek score 66.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 688
variable_sites: 24
pi: 0.00775
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGGATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	57.6	58.5	736-808 bp	1.0	79.6
psbM-trnD-GUC_p2	AGGATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	50.0	50.0	57.6	57.0	737-809 bp	1.0	75.4
psbM-trnD-GUC_p3	AGGATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	50.0	52.6	57.6	57.8	735-807 bp	1.0	73.5
psbM-trnD-GUC_p4	GGAACTAGGATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	47.6	50.0	57.7	58.5	742-814 bp	1.0	72.0
psbM-trnD-GUC_p5	AGGATGAACAGTGCAGTAGC	AGTTCAATTGGTCAGAGCACC	50.0	47.6	57.6	58.5	738-810 bp	1.0	71.4