Hotspot

rps16-trnQ-UUG

LSC · 6018-7293 · 1276 bp

Back to Libanotis

Pi 0.012
MarkerSeek score 55.40
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1276
variable_sites
59
pi
0.012
unique_haplotype_count
12
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 TGCTTTCTACCACAGCGTTT TGGAAGTATAGATGGGGCGT 45.0 50.0 58.0 57.9 937-1441 bp 1.0 86.6
rps16-trnQ-UUG_p2 TTGCTTTCTACCACAGCGTT TGGAAGTATAGATGGGGCGT 45.0 50.0 58.0 57.9 938-1442 bp 1.0 86.6
rps16-trnQ-UUG_p3 TGCTTTCTACCACAGCGTTT CTTACAGCAGCTTGCCAAAC 45.0 50.0 58.0 57.9 1453-1955 bp 1.0 86.5
rps16-trnQ-UUG_p4 TTGCTTTCTACCACAGCGTT CTTACAGCAGCTTGCCAAAC 45.0 50.0 58.0 57.9 1454-1956 bp 1.0 86.5
rps16-trnQ-UUG_p5 GATTCCCGCGTGATACACTT CTTACAGCAGCTTGCCAAAC 50.0 50.0 58.1 57.9 1810-2312 bp 1.0 86.1