Hotspot

trnK-UUU-rps16

LSC · 4204-4913 · 710 bp

Back to Libanotis

Pi 0.00837
MarkerSeek score 67.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
710
variable_sites
29
pi
0.00837
unique_haplotype_count
12
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TCCCTCGCTTCATCCAAATG AGGAACTGTTCGGGATCTTT 50.0 45.0 58.0 56.5 834-886 bp 1.0 71.8
trnK-UUU-rps16_p2 CCGATGGTATGGACGAATCC AGGAACTGTTCGGGATCTTT 55.0 45.0 57.9 56.5 851-903 bp 1.0 71.4
trnK-UUU-rps16_p3 CGATGGTATGGACGAATCCC AGGAACTGTTCGGGATCTTT 55.0 45.0 57.9 56.5 850-902 bp 1.0 71.4
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AGGAACTGTTCGGGATCTTT 50.0 45.0 58.2 56.5 789-841 bp 1.0 71.0
trnK-UUU-rps16_p5 AAAGCCGAGTACTCTACCGT AGGAACTGTTCGGGATCTTT 50.0 45.0 58.2 56.5 790-842 bp 1.0 70.9