Hotspot

ycf23

Genome · 51321-52118 · 798 bp

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Pi 0.0576

MarkerSeek score 73.50

Primer available yes

Label ycf23

Feature Metadata

label_name: ycf23
feature_type: gene
region: Genome
length_bp: 798
variable_sites: 46
pi: 0.0576
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf23_p1	ATGCTTTAGCCTTTGGAGTT	AGCTAATACATGACATCCAATCA	40.0	34.8	55.2	55.5	857 bp	1.0	44.4
ycf23_p2	ATGCTTTAGCCTTTGGAGTT	GCTAATACATGACATCCAATCATCA	40.0	36.0	55.2	57.3	856 bp	1.0	44.4
ycf23_p3	ATGCTTTAGCCTTTGGAGTT	ACATGACATCCAATCATCAT	40.0	35.0	55.2	52.3	850 bp	1.0	44.4
ycf23_p4	ATGCTTTAGCCTTTGGAGTT	AAGCTAATACATGACATCCAATCA	40.0	33.3	55.2	56.2	858 bp	1.0	44.4
ycf23_p5	ATGCTTTAGCCTTTGGAGTT	ACATGACATCCAATCATCATT	40.0	33.3	55.2	53.2	850 bp	1.0	44.4