Hotspot

trpA

Genome · 38404-39198 · 795 bp

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Pi 0.0491

MarkerSeek score 72.00

Primer available yes

Label trpA

Feature Metadata

label_name: trpA
feature_type: gene
region: Genome
length_bp: 795
variable_sites: 39
pi: 0.0491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trpA_p1	CGATTAGTTCCGGGTTCGAG	ATACGGCATGGCAAGAACAT	55.0	45.0	58.2	57.9	1301 bp	1.0	81.9
trpA_p2	CGATTAGTTCCGGGTTCGAG	CGAAGCTACGGACAACAAGA	55.0	50.0	58.2	57.9	1242 bp	1.0	81.9
trpA_p3	TTTGAGTCGTGTCTTGCCTC	ATACGGCATGGCAAGAACAT	50.0	45.0	58.1	57.9	1627 bp	0.5	58.5
trpA_p4	TTTGAGTCGTGTCTTGCCTC	CGAAGCTACGGACAACAAGA	50.0	50.0	58.1	57.9	1568 bp	0.5	58.4
trpA_p5	TTTGAGTCGTGTCTTGCCTC	CAATTAAGGCCAGGGCTGAT	50.0	50.0	58.1	57.9	1685 bp	0.5	58.3