Hotspot

tilS

Genome · 37163-38170 · 1008 bp

Back to Liagora

Pi 0.0605

MarkerSeek score 78.50

Primer available yes

Label tilS

Feature Metadata

label_name: tilS
feature_type: gene
region: Genome
length_bp: 1008
variable_sites: 61
pi: 0.0605
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tilS_p1	GCCTTATTGATCAATCGTATGCC	ACTAAATTCATATCACTAGCACC	43.5	34.8	58.0	54.0	1064 bp	1.0	44.6
tilS_p2	GCCTTATTGATCAATCGTATGCC	AACTAAATTCATATCACTAGCACC	43.5	33.3	58.0	54.8	1065 bp	1.0	44.6
tilS_p3	GCCTTATTGATCAATCGTATGCC	ATCAAGACCTAACTCAAAACT	43.5	33.3	58.0	52.4	1082 bp	1.0	44.5
tilS_p4	TGCCTTATTGATCAATCGTATGC	ATCAAGACCTAACTCAAAACT	39.1	33.3	57.4	52.4	1083 bp	1.0	44.5
tilS_p5	GCCTTATTGATCAATCGTATGCC	TCAAGACCTAACTCAAAACTAA	43.5	31.8	58.0	52.7	1081 bp	1.0	44.5