Hotspot

syfB

Genome · 170895-172895 · 2001 bp

Back to Liagora

Pi 0.0575

MarkerSeek score 72.80

Primer available yes

Label syfB

Feature Metadata

label_name: syfB
feature_type: gene
region: Genome
length_bp: 2001
variable_sites: 115
pi: 0.0575
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
syfB_p1	GTGCCACAGAAAAATACCGC	AGGTACTGCAACATTTCGCT	50.0	45.0	58.0	57.8	2639 bp	1.0	82.5
syfB_p2	GTGCCACAGAAAAATACCGC	GAAATCACGGGCATGTACCT	50.0	50.0	58.0	58.0	2575 bp	0.5	59.5
syfB_p3	GCTTATGGCGGAAGAACAGA	GAAATCACGGGCATGTACCT	50.0	50.0	58.0	58.0	2343 bp	0.5	59.5
syfB_p4	ATGGCGGAAGAACAGAATCC	GAAATCACGGGCATGTACCT	50.0	50.0	58.0	58.0	2339 bp	0.5	59.3
syfB_p5	GGAAGAACAGAATCCGGCTT	GAAATCACGGGCATGTACCT	50.0	50.0	57.9	58.0	2334 bp	0.5	58.8