Hotspot

ompR

Genome · 181913-182644 · 732 bp

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Pi 0.041

MarkerSeek score 68.10

Primer available yes

Label ompR

Feature Metadata

label_name: ompR
feature_type: gene
region: Genome
length_bp: 732
variable_sites: 30
pi: 0.041
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ompR_p1	GAATGTCCCATGCTGTTTGC	GCTCACTGATCGGACAACAA	50.0	50.0	58.0	58.2	1291 bp	0.5	58.4
ompR_p2	ATGCTGTTTGCTGGAGTTGA	GCTCACTGATCGGACAACAA	45.0	50.0	58.0	58.2	1282 bp	0.5	58.2
ompR_p3	TGCTGTTTGCTGGAGTTGAT	GCTCACTGATCGGACAACAA	45.0	50.0	58.0	58.2	1281 bp	0.5	58.2
ompR_p4	AAGTCACCTTGTGCTTCAGG	GCTCACTGATCGGACAACAA	50.0	50.0	58.0	58.2	1330 bp	0.5	58.1
ompR_p5	CATGCTGTTTGCTGGAGTTG	GCTCACTGATCGGACAACAA	50.0	50.0	57.9	58.2	1283 bp	0.5	57.9