Hotspot

dnaB

Genome · 161048-162817 · 1770 bp

Back to Liagora

Pi 0.0508

MarkerSeek score 72.20

Primer available yes

Label dnaB

Feature Metadata

label_name: dnaB
feature_type: gene
region: Genome
length_bp: 1770
variable_sites: 90
pi: 0.0508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaB_p1	TCAGTCCACTGCTCTACCAA	TAGTACAAGTGGCCCAAGGA	50.0	50.0	58.0	58.0	2323 bp	1.0	83.7
dnaB_p2	TCCACTGCTCTACCAACTGA	TAGTACAAGTGGCCCAAGGA	50.0	50.0	58.0	58.0	2319 bp	1.0	83.7
dnaB_p3	TTCAGTCCACTGCTCTACCA	TAGTACAAGTGGCCCAAGGA	50.0	50.0	58.0	58.0	2324 bp	1.0	83.7
dnaB_p4	TCAGTCCACTGCTCTACCAA	CACTCGACCACCTTTCCAAA	50.0	50.0	58.0	58.0	2507 bp	1.0	83.5
dnaB_p5	TCCACTGCTCTACCAACTGA	CACTCGACCACCTTTCCAAA	50.0	50.0	58.0	58.0	2503 bp	1.0	83.5