Hotspot

accB

Genome · 123041-123538 · 498 bp

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Pi 0.0542

MarkerSeek score 76.50

Primer available yes

Label accB

Feature Metadata

label_name: accB
feature_type: gene
region: Genome
length_bp: 498
variable_sites: 27
pi: 0.0542
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accB_p1	TCTTCATTCAATCAAGGAGTT	TCTGGATGATCTACAATCTTTT	33.3	31.8	52.7	52.6	588 bp	1.0	43.7
accB_p2	TCTTCATTCAATCAAGGAGTT	TTCTGGATGATCTACAATCTTT	33.3	31.8	52.7	52.6	589 bp	1.0	43.7
accB_p3	TCTTCATTCAATCAAGGAGTT	TTTCTGGATGATCTACAATCTT	33.3	31.8	52.7	52.6	590 bp	1.0	43.7
accB_p4	TTCTTCATTCAATCAAGGAGT	TCTGGATGATCTACAATCTTTT	33.3	31.8	52.7	52.6	589 bp	1.0	43.7
accB_p5	TTCTTCATTCAATCAAGGAGT	TTCTGGATGATCTACAATCTTT	33.3	31.8	52.7	52.6	590 bp	1.0	43.7