Hotspot

ORF_8

Genome · 68233-68400 · 168 bp

Back to Liagora

Pi 0.0595

MarkerSeek score 67.50

Primer available yes

Label ORF_8

Feature Metadata

label_name: ORF_8
feature_type: gene
region: Genome
length_bp: 168
variable_sites: 10
pi: 0.0595
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF_8_p1	TGCACCAGTGGTTGATGAAA	CAAAGTGCAGGCTTAAGTGC	45.0	50.0	57.9	57.9	971-972 bp	1.0	83.6
ORF_8_p2	TTGCACCAGTGGTTGATGAA	CAAAGTGCAGGCTTAAGTGC	45.0	50.0	57.9	57.9	972-973 bp	1.0	83.6
ORF_8_p3	TGCACCAGTGGTTGATGAAA	CCGCAGACCAATCACATGTA	45.0	50.0	57.9	58.0	768 bp	0.5	59.3
ORF_8_p4	TTGCACCAGTGGTTGATGAA	CCGCAGACCAATCACATGTA	45.0	50.0	57.9	58.0	769 bp	0.5	59.3
ORF_8_p5	TGCACCAGTGGTTGATGAAA	GGCTTAAGTGCGGTGAGTTA	45.0	50.0	57.9	57.9	963 bp	0.5	58.8