Hotspot

ORF_4

Genome · 73013-75259 · 2247 bp

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Pi 0.0641

MarkerSeek score 75.40

Primer available yes

Label ORF_4

Feature Metadata

label_name: ORF_4
feature_type: gene
region: Genome
length_bp: 2247
variable_sites: 144
pi: 0.0641
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF_4_p1	TGTTTGATATCGGGACACGC	TCCGAGCTGAATGAAACACA	50.0	45.0	58.4	57.5	2957-2958 bp	1.0	77.5
ORF_4_p2	TGTTTGATATCGGGACACGC	TGTCCGAGCTGAATGAAACA	50.0	45.0	58.4	57.5	2959-2960 bp	1.0	77.5
ORF_4_p3	ATTCAGCCTGTGGATTCAGC	TCCGAGCTGAATGAAACACA	50.0	45.0	58.2	57.5	2917 bp	0.5	53.7
ORF_4_p4	ATTCAGCCTGTGGATTCAGC	TGTCCGAGCTGAATGAAACA	50.0	45.0	58.2	57.5	2919 bp	0.5	53.7
ORF_4_p5	ATTCAGCCTGTGGATTCAGC	GTCCGAGCTGAATGAAACAC	50.0	50.0	58.2	57.1	2918 bp	0.5	51.0