Hotspot

trnY-trnV

Genome · 157216-157289 · 74 bp

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Pi 0.149

MarkerSeek score 76.20

Primer available yes

Label trnY-trnV

Feature Metadata

label_name: trnY-trnV
feature_type: igs
region: Genome
length_bp: 74
variable_sites: 11
pi: 0.149
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-trnV_p1	AAGCCAGCGGATTTACAGTC	GTGGCTAGAGTACCACGTTG	50.0	55.0	58.3	58.0	183 bp	1.0	82.7
trnY-trnV_p2	AAGCCAGCGGATTTACAGTC	TGGCTAGAGTACCACGTTGA	50.0	50.0	58.3	58.1	182 bp	1.0	82.1
trnY-trnV_p3	AAGCCAGCGGATTTACAGTC	CTCAGGTGGCTAGAGTACCA	50.0	55.0	58.3	57.9	188 bp	1.0	81.4
trnY-trnV_p4	AAGCCAGCGGATTTACAGTC	ACTCAGGTGGCTAGAGTACC	50.0	55.0	58.3	57.9	189 bp	1.0	81.4
trnY-trnV_p5	AAGCCAGCGGATTTACAGTC	ACGTTGACATCGTGGAAGTC	50.0	50.0	58.3	58.2	169 bp	1.0	81.4