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rps9-rpl31

Genome · 144425-144446 · 22 bp

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Pi 0.1

MarkerSeek score 77.30

Primer available yes

Label rps9-rpl31

Feature Metadata

label_name: rps9-rpl31
feature_type: igs
region: Genome
length_bp: 22
variable_sites: 2
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 77.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps9-rpl31_p1	AAAGCACGAAAAGCTCCTCA	TGGGTGAATATTTTGTTTTGCCA	45.0	34.8	58.0	57.7	80-82 bp	1.0	57.3
rps9-rpl31_p2	AAAGCACGAAAAGCTCCTCA	CTGGGTGAATATTTTGTTTTGCC	45.0	39.1	58.0	57.1	81-83 bp	1.0	52.8
rps9-rpl31_p3	AAAGCACGAAAAGCTCCTCA	TGGGTGAATATTTTGTTTTGCC	45.0	36.4	58.0	56.0	80-82 bp	1.0	51.9
rps9-rpl31_p4	AAGCACGAAAAGCTCCTCAG	TGGGTGAATATTTTGTTTTGCCA	50.0	34.8	58.5	57.7	81 bp	0.5	29.8
rps9-rpl31_p5	AAAGCACGAAAAGCTCCTCA	GGGTGAATATTTTGTTTTGCCA	45.0	36.4	58.0	56.0	81 bp	0.5	28.0