Hotspot

rpl20-rpl35

Genome · 112091-112103 · 13 bp

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Pi 0.154
MarkerSeek score 76.00
Primer available yes
Label rpl20-rpl35

Feature Metadata

label_name
rpl20-rpl35
feature_type
igs
region
Genome
length_bp
13
variable_sites
2
pi
0.154
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rpl35_p1 ACCTCTTTTTACACGTGTCA TCTGGTGATATGCAACGTAT 40.0 40.0 54.9 54.3 82 bp 1.0 42.0
rpl20-rpl35_p2 ACCTCTTTTTACACGTGTCA ATTCTGGTGATATGCAACGT 40.0 40.0 54.9 55.1 84 bp 1.0 41.9
rpl20-rpl35_p3 ACCTCTTTTTACACGTGTCAT ATTCTGGTGATATGCAACGT 38.1 40.0 55.5 55.1 84 bp 1.0 41.9
rpl20-rpl35_p4 ACCTCTTTTTACACGTGTCA TTCTGGTGATATGCAACGTA 40.0 40.0 54.9 54.5 83 bp 1.0 41.9
rpl20-rpl35_p5 TACCTCTTTTTACACGTGTCA ATTCTGGTGATATGCAACGT 38.1 40.0 54.9 55.1 85 bp 1.0 41.9