Hotspot

trnG-trnM

Genome · 70166-70213 · 48 bp

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Pi 0.292

MarkerSeek score 76.50

Primer available yes

Label trnG-trnM

Feature Metadata

label_name: trnG-trnM
feature_type: igs
region: Genome
length_bp: 48
variable_sites: 14
pi: 0.292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-trnM_p1	TTCCAAGCTAATGATGCGGG	TCCATAAAAGGACGTCGCTG	50.0	50.0	58.3	58.0	130 bp	1.0	85.4
trnG-trnM_p2	GCTAATGATGCGGGTTCGAT	TCCATAAAAGGACGTCGCTG	50.0	50.0	58.5	58.0	124 bp	1.0	84.1
trnG-trnM_p3	TTCCAAGCTAATGATGCGGG	TAAAAGGACGTCGCTGGTTC	50.0	50.0	58.3	58.2	126 bp	1.0	83.7
trnG-trnM_p4	TTCCAAGCTAATGATGCGGG	CATAAAAGGACGTCGCTGGT	50.0	50.0	58.3	58.3	128 bp	1.0	83.2
trnG-trnM_p5	CCAAGCTAATGATGCGGGTT	TCCATAAAAGGACGTCGCTG	50.0	50.0	58.6	58.0	128 bp	1.0	83.1