Hotspot

trnC-cbbX

Genome · 64215-64277 · 63 bp

Back to Liagora

Pi 0.0952

MarkerSeek score 77.70

Primer available yes

Label trnC-cbbX

Feature Metadata

label_name: trnC-cbbX
feature_type: igs
region: Genome
length_bp: 63
variable_sites: 6
pi: 0.0952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-cbbX_p1	CCTTACCACTTGGCCATACC	TATTTGCAGGCGAATTCGGA	55.0	45.0	57.9	58.0	112 bp	1.0	83.6
trnC-cbbX_p2	TCGAACTGGGGGTAAAGGAT	TATTTGCAGGCGAATTCGGA	50.0	45.0	58.0	58.0	145 bp	1.0	82.7
trnC-cbbX_p3	TTGCAATCCTCTGCCTTACC	TATTTGCAGGCGAATTCGGA	50.0	45.0	57.9	58.0	125 bp	1.0	82.6
trnC-cbbX_p4	CCTTACCACTTGGCCATACC	GAGTCGTTTATTTGCAGGCG	55.0	50.0	57.9	57.8	120 bp	1.0	82.3
trnC-cbbX_p5	TCGAACTGGGGGTAAAGGAT	GAGTCGTTTATTTGCAGGCG	50.0	50.0	58.0	57.8	153 bp	1.0	81.6