Hotspot

trnR-trnV

Genome · 32021-32046 · 26 bp

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Pi 0.115

MarkerSeek score 78.20

Primer available yes

Label trnR-trnV

Feature Metadata

label_name: trnR-trnV
feature_type: igs
region: Genome
length_bp: 26
variable_sites: 3
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-trnV_p1	GGTCCCTGTCCTAATCCCTT	CTGGTTCAAGTCCAGTACCG	55.0	55.0	58.1	57.9	84 bp	1.0	81.4
trnR-trnV_p2	AAGGTCCCTGTCCTAATCCC	CTGGTTCAAGTCCAGTACCG	55.0	55.0	58.1	57.9	86 bp	1.0	81.3
trnR-trnV_p3	AAGGTCCCTGTCCTAATCCC	TTACAAGCAGGTGGTCACTG	55.0	50.0	58.1	57.7	103 bp	1.0	79.5
trnR-trnV_p4	GGTCCCTGTCCTAATCCCTT	TTACAAGCAGGTGGTCACTG	55.0	50.0	58.1	57.7	101 bp	1.0	79.5
trnR-trnV_p5	GGTCCCTGTCCTAATCCCTT	CTATAAAGTTGGGTGGGCGG	55.0	55.0	58.1	58.3	146 bp	1.0	78.2