Hotspot

rpl32-trnL-UAG

SSC · 104909-105610 · 702 bp

Back to Leymus

Pi 0.0172
MarkerSeek score 52.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
702
variable_sites
35
pi
0.0172
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.92
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 777-826 bp 1.0 86.7
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.1 58.0 776-825 bp 1.0 86.7
rpl32-trnL-UAG_p3 TTCCAGCGGTAATGAGCATC TTCGAACCGAGATGCTTGAG 50.0 50.0 58.0 58.0 805-854 bp 0.769231 78.1
rpl32-trnL-UAG_p4 TTCCAGCGGTAATGAGCATC GGATTCGAACCGAGATGCTT 50.0 50.0 58.0 58.1 808-857 bp 0.769231 77.6
rpl32-trnL-UAG_p5 TTCCAGCGGTAATGAGCATC GATCCGCACAAAAGAGTTGC 50.0 50.0 58.0 58.0 1351-1400 bp 0.769231 76.2