Hotspot

ndhF-rpl32

SSC · 103802-104716 · 915 bp

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Pi 0.0106
MarkerSeek score 53.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
915
variable_sites
30
pi
0.0106
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.98
markerseek_score
53.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGAAGTGGGAGAAGAGGGAT TTGACATTGAAGTACGTTTTT 55.0 28.6 58.1 52.2 979-1002 bp 1.0 44.4
ndhF-rpl32_p2 GGAAGTGGGAGAAGAGGGAT TTTGACATTGAAGTACGTTTT 55.0 28.6 58.1 52.2 980-1003 bp 1.0 44.4
ndhF-rpl32_p3 GGAAGTGGGAGAAGAGGGAT TTTTGACATTGAAGTACGTTT 55.0 28.6 58.1 52.2 981-1004 bp 1.0 44.4
ndhF-rpl32_p4 GGAAGTGGGAGAAGAGGGAT TTTTTGACATTGAAGTACGTT 55.0 28.6 58.1 52.2 982-1005 bp 1.0 44.4
ndhF-rpl32_p5 GGAAGTGGGAGAAGAGGGAT TCCAAATATTTTTACGAATACGCT 55.0 29.2 58.1 55.3 1006-1029 bp 1.0 44.3