Hotspot

rpl22-rps19

IRb · 80668-80745 · 78 bp

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Pi 0.0438
MarkerSeek score 68.40
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
IRb
length_bp
78
variable_sites
11
pi
0.0438
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TCTTCTTCTTTATCCTAGGGA CAAATTGGGGGAATTCGTGC 38.1 50.0 52.4 58.0 204-207 bp 1.0 45.8
rpl22-rps19_p2 ACTTTTCTTCTTCTTTATCCTAGGGA CAAATTGGGGGAATTCGTGC 34.6 50.0 57.5 58.0 209-212 bp 1.0 45.7
rpl22-rps19_p3 ACTTTTCTTCTTCTTTATCCTAGGGA GCAAATTGGGGGAATTCGTG 34.6 50.0 57.5 58.0 210-213 bp 1.0 45.7
rpl22-rps19_p4 CACTTTTCTTCTTCTTTATCCTAGGG CAAATTGGGGGAATTCGTGC 38.5 50.0 57.5 58.0 210-213 bp 1.0 45.7
rpl22-rps19_p5 CACTTTTCTTCTTCTTTATCCTAGGG GCAAATTGGGGGAATTCGTG 38.5 50.0 57.5 58.0 211-214 bp 1.0 45.6