Hotspot

petG-trnW-CCA

LSC · 64043-64163 · 121 bp

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Pi 0.0307
MarkerSeek score 63.50
Primer available yes
Label petG-trnW-CCA

Feature Metadata

label_name
petG-trnW-CCA
feature_type
igs
region
LSC
length_bp
121
variable_sites
12
pi
0.0307
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.95
markerseek_score
63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-trnW-CCA_p1 GGGATTATTCGTGACTGCGT CAAAACCCGATGTCGTAGGT 50.0 50.0 58.1 57.9 214-218 bp 1.0 85.5
petG-trnW-CCA_p2 GGGATTATTCGTGACTGCGT AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.1 58.0 250-254 bp 1.0 85.1
petG-trnW-CCA_p3 AGCGGGATTATTCGTGACTG CAAAACCCGATGTCGTAGGT 50.0 50.0 57.8 57.9 217-221 bp 1.0 84.1
petG-trnW-CCA_p4 GCGTATTTGCAATACAGGCG AGCGCTCTTAGTTCAGTTCG 50.0 50.0 58.3 58.0 234-238 bp 1.0 83.9
petG-trnW-CCA_p5 AGCGGGATTATTCGTGACTG AGCGCTCTTAGTTCAGTTCG 50.0 50.0 57.8 58.0 253-257 bp 1.0 83.7