Hotspot

rbcL-psaI

LSC · 55891-57532 · 1642 bp

Pi 0.0164

MarkerSeek score 53.20

Primer available yes

Label rbcL-psaI

Feature Metadata

label_name: rbcL-psaI
feature_type: igs
region: LSC
length_bp: 1642
variable_sites: 65
pi: 0.0164
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psaI_p1	TCTTCACATTCACCGTGCAA	GTCATTGCAATTGCCGGAAA	45.0	45.0	58.0	57.9	1474-2286 bp	1.0	85.9
rbcL-psaI_p2	CCGAAATCTTTGGGGACGAT	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.0	57.9	1172-1978 bp	0.923077	83.0
rbcL-psaI_p3	TCTTCACATTCACCGTGCAA	AACTCGCTATACCTGGCTCT	45.0	50.0	58.0	57.9	1660-2472 bp	0.307692	56.5
rbcL-psaI_p4	TTCTTCACATTCACCGTGCA	AACTCGCTATACCTGGCTCT	45.0	50.0	58.0	57.9	1661-2473 bp	0.307692	56.5
rbcL-psaI_p5	CCGAAATCTTTGGGGACGAT	AACTCGCTATACCTGGCTCT	50.0	50.0	58.0	57.9	1358-2165 bp	0.230769	53.1