Hotspot

trnC-GCA-rpoB

LSC · 18188-19345 · 1158 bp

Back to Leymus

Pi 0.00929
MarkerSeek score 55.90
Primer available yes
Label trnC-GCA-rpoB

Feature Metadata

label_name
trnC-GCA-rpoB
feature_type
igs
region
LSC
length_bp
1158
variable_sites
31
pi
0.00929
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-rpoB_p1 AAAGGATTTGCAGTCCCCTG ATTCCCTCGTTTCCATTCCG 50.0 50.0 58.1 58.0 1225-1239 bp 1.0 82.1
trnC-GCA-rpoB_p2 AAAGGATTTGCAGTCCCCTG TGTGGACATTCCCTCGTTTC 50.0 50.0 58.1 57.8 1232-1246 bp 1.0 81.0
trnC-GCA-rpoB_p3 AAAGGATTTGCAGTCCCCTG CCGGGTATTGTGGACATTCC 50.0 55.0 58.1 58.3 1240-1254 bp 1.0 79.8
trnC-GCA-rpoB_p4 AAAGGATTTGCAGTCCCCTG CGGGTATTGTGGACATTCCC 50.0 55.0 58.1 58.3 1239-1253 bp 1.0 79.8
trnC-GCA-rpoB_p5 AAAGGATTTGCAGTCCCCTG AAGTTCTTCTGCCAAGGCTT 50.0 45.0 58.1 57.6 1304-1318 bp 1.0 79.3