Hotspot

trnY-GUA-trnD-GUC

LSC · 15469-15827 · 359 bp

Back to Leymus

Pi 0.0142
MarkerSeek score 55.80
Primer available yes
Label trnY-GUA-trnD-GUC

Feature Metadata

label_name
trnY-GUA-trnD-GUC
feature_type
igs
region
LSC
length_bp
359
variable_sites
18
pi
0.0142
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnY-GUA-trnD-GUC_p1 AATTCTTCCTGGGTCGATGC CGGTGCTCTGACCAATTGAA 50.0 50.0 58.0 58.5 477-487 bp 1.0 80.5
trnY-GUA-trnD-GUC_p2 GGGGACGGACTGTAAATTCG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.4 58.5 444-454 bp 1.0 78.4
trnY-GUA-trnD-GUC_p3 GGGACGGACTGTAAATTCGT CGGTGCTCTGACCAATTGAA 50.0 50.0 57.6 58.5 443-453 bp 1.0 78.2
trnY-GUA-trnD-GUC_p4 AATTCTTCCTGGGTCGATGC GGTGCTCTGACCAATTGAAC 50.0 50.0 58.0 57.0 476-486 bp 1.0 76.4
trnY-GUA-trnD-GUC_p5 GATGCCCGAGTGGTTAATGG CGGTGCTCTGACCAATTGAA 55.0 50.0 58.7 58.5 462-472 bp 1.0 75.5