Hotspot

trnT-GGU-trnE-UUC

LSC · 14724-15252 · 529 bp

Back to Leymus

Pi 0.00954
MarkerSeek score 54.30
Primer available yes
Label trnT-GGU-trnE-UUC

Feature Metadata

label_name
trnT-GGU-trnE-UUC
feature_type
igs
region
LSC
length_bp
529
variable_sites
18
pi
0.00954
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnE-UUC_p1 AGCCCTTTTAACTCAGTGGT GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.7 58.3 612-655 bp 1.0 73.3
trnT-GGU-trnE-UUC_p2 AGCCCTTTTAACTCAGTGGT TGTCCTGAACCACTAGACGA 45.0 50.0 56.7 57.7 591-634 bp 1.0 73.3
trnT-GGU-trnE-UUC_p3 AGGCATAAGTCATCGGTTCA GCTGCCTCCTTGAAAGAGAG 45.0 55.0 56.6 58.3 575-618 bp 1.0 73.3
trnT-GGU-trnE-UUC_p4 AGGCATAAGTCATCGGTTCA TGTCCTGAACCACTAGACGA 45.0 50.0 56.6 57.7 554-597 bp 1.0 73.2
trnT-GGU-trnE-UUC_p5 TGGTAAGGCATAAGTCATCGG GCTGCCTCCTTGAAAGAGAG 47.6 55.0 57.5 58.3 580-623 bp 1.0 72.5