Hotspot

trnG-GCC-trnM-CAU

LSC · 12167-12614 · 448 bp

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Pi 0.0193
MarkerSeek score 53.50
Primer available yes
Label trnG-GCC-trnM-CAU

Feature Metadata

label_name
trnG-GCC-trnM-CAU
feature_type
igs
region
LSC
length_bp
448
variable_sites
24
pi
0.0193
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-trnM-CAU_p1 GCGAGCGTAGTTCAATGGTA CGAGGCTCATAACCTTGAGG 50.0 55.0 58.1 57.8 563-566 bp 1.0 82.3
trnG-GCC-trnM-CAU_p2 GCGAGCGTAGTTCAATGGTA CAGTTTGGTAGCTCACGAGG 50.0 55.0 58.1 58.3 578-581 bp 1.0 81.8
trnG-GCC-trnM-CAU_p3 TAGCGAGCGTAGTTCAATGG CGAGGCTCATAACCTTGAGG 50.0 55.0 57.8 57.8 565-568 bp 1.0 81.3
trnG-GCC-trnM-CAU_p4 GCGAGCGTAGTTCAATGGTA GAGCGGAGTAGAGCAGTTTG 50.0 55.0 58.1 58.4 591-594 bp 1.0 81.0
trnG-GCC-trnM-CAU_p5 TAGCGAGCGTAGTTCAATGG CAGTTTGGTAGCTCACGAGG 50.0 55.0 57.8 58.3 580-583 bp 1.0 80.8