Hotspot

trnK-UUU-rps16

LSC · 3943-4508 · 566 bp

Back to Leymus

Pi 0.0154
MarkerSeek score 48.70
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
566
variable_sites
25
pi
0.0154
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AGTCGCACTTAAAAGCCGAG AGAAAAAGAAAGAACTTTGAGT 50.0 27.3 58.6 52.1 635-650 bp 1.0 43.9
trnK-UUU-rps16_p2 AGTCGCACTTAAAAGCCGAG AGAAAAAGAAAGAACTTTGAGTT 50.0 26.1 58.6 53.0 635-650 bp 1.0 43.9
trnK-UUU-rps16_p3 TCGCACTTAAAAGCCGAGTA AGAAAAAGAAAGAACTTTGAGT 45.0 27.3 57.3 52.1 633-648 bp 1.0 43.9
trnK-UUU-rps16_p4 AGTCGCACTTAAAAGCCGAG AAGAAAAAGAAAGAACTTTGAGT 50.0 26.1 58.6 53.0 636-651 bp 1.0 43.8
trnK-UUU-rps16_p5 TAGTCGCACTTAAAAGCCGA AGAAAAAGAAAGAACTTTGAGT 45.0 27.3 57.3 52.1 636-651 bp 1.0 43.8