Hotspot

ndhF-rpl32

SSC · 114716-115639 · 924 bp

Back to Leucojum

Pi 0.0189
MarkerSeek score 65.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
924
variable_sites
17
pi
0.0189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCTGTCGGAATAAGCA AGAAAAAGCTTTTATGGCAGC 45.0 38.1 57.8 55.6 1100-1122 bp 1.0 54.7
ndhF-rpl32_p2 TGTTGCTGTCGGAATAAGCA AAGAAAAAGCTTTTATGGCAGC 45.0 36.4 57.8 56.4 1101-1123 bp 1.0 52.6
ndhF-rpl32_p3 TGTTGCTGTCGGAATAAGCA TGGCAGCTAAATATCCCTTT 45.0 40.0 57.8 54.4 1086-1108 bp 1.0 52.4
ndhF-rpl32_p4 TGTTGCTGTCGGAATAAGCA TGGCAGCTAAATATCCCTTTT 45.0 38.1 57.8 55.2 1086-1108 bp 1.0 51.3
ndhF-rpl32_p5 TGTTGCTGTCGGAATAAGCA TGGCAGCTAAATATCCCTTTTTC 45.0 39.1 57.8 57.1 1086-1108 bp 1.0 50.8