Hotspot

trnW-CCA-trnP-UGG

LSC · 68298-68467 · 170 bp

Back to Leucojum

Pi 0.0414
MarkerSeek score 73.60
Primer available yes
Label trnW-CCA-trnP-UGG

Feature Metadata

label_name
trnW-CCA-trnP-UGG
feature_type
igs
region
LSC
length_bp
170
variable_sites
7
pi
0.0414
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnW-CCA-trnP-UGG_p1 GCGTTCTACCGAACTGAACT GTCACGGGTTCAAATCCTGT 50.0 50.0 57.9 58.1 225-226 bp 1.0 81.5
trnW-CCA-trnP-UGG_p2 GCGTTCTACCGAACTGAACT CACGGGTTCAAATCCTGTCA 50.0 50.0 57.9 58.1 223-224 bp 1.0 81.5
trnW-CCA-trnP-UGG_p3 GCGTTCTACCGAACTGAACT TGTCACGGGTTCAAATCCTG 50.0 50.0 57.9 58.1 226-227 bp 1.0 81.5
trnW-CCA-trnP-UGG_p4 GCGTTCTACCGAACTGAACT GGGTACAAAATGTCACGGGT 50.0 50.0 57.9 58.1 236-237 bp 1.0 81.4
trnW-CCA-trnP-UGG_p5 GCGTTCTACCGAACTGAACT TGGGTACAAAATGTCACGGG 50.0 50.0 57.9 58.1 237-238 bp 1.0 81.4