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petA-psbJ

LSC · 64843-66002 · 1160 bp

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Pi 0.0347

MarkerSeek score 65.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 22
pi: 0.0347
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.55
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCCTTTTGTTCTTCTTGGC	CCGGATTGGGTTCATCTCTG	50.0	55.0	57.8	58.0	759-1285 bp	1.0	84.2
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	TCCGGATTGGGTTCATCTCT	50.0	50.0	57.8	58.1	760-1286 bp	1.0	83.5
petA-psbJ_p3	CGTCCAAGGCCTTTTGTTCT	CCGGATTGGGTTCATCTCTG	50.0	55.0	58.7	58.0	766-1292 bp	1.0	79.9
petA-psbJ_p4	CGTCCAAGGCCTTTTGTTCT	TCCGGATTGGGTTCATCTCT	50.0	50.0	58.7	58.1	767-1293 bp	1.0	79.3
petA-psbJ_p5	GATCCATTACGCGTCCAAGG	CCGGATTGGGTTCATCTCTG	55.0	55.0	58.8	58.0	777-1303 bp	1.0	79.1