Hotspot

ycf4-cemA

LSC · 62180-62977 · 798 bp

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Pi 0.0296

MarkerSeek score 64.30

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 798
variable_sites: 23
pi: 0.0296
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TGCGCGTACCAATTGAAGTA	CAACCATGGGTCGAGTGAAA	45.0	50.0	57.9	58.1	1310-1331 bp	1.0	81.4
ycf4-cemA_p2	TATGGGATCGCGGGTCTATT	CAACCATGGGTCGAGTGAAA	50.0	50.0	58.1	58.1	1635-1656 bp	1.0	81.0
ycf4-cemA_p3	TATGGGATCGCGGGTCTATT	GGGTCGAGTGAAATCCGATC	50.0	55.0	58.1	58.1	1628-1649 bp	1.0	80.7
ycf4-cemA_p4	TATGGGATCGCGGGTCTATT	GGTCGAGTGAAATCCGATCC	50.0	55.0	58.1	58.1	1627-1648 bp	1.0	80.7
ycf4-cemA_p5	GGGTCCCGAAAAGCAAGTAA	CAACCATGGGTCGAGTGAAA	50.0	50.0	58.1	58.1	1797-1818 bp	1.0	80.6