Hotspot

trnD-GUC-trnY-GUA

LSC · 31941-32311 · 371 bp

Back to Leucojum

Pi 0.0291

MarkerSeek score 66.10

Primer available yes

Label trnD-GUC-trnY-GUA

Feature Metadata

label_name: trnD-GUC-trnY-GUA
feature_type: igs
region: LSC
length_bp: 371
variable_sites: 10
pi: 0.0291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-trnY-GUA_p1	GTGCTCTGACCGATTGAACT	GGGGACGGACTGTAAATTCG	50.0	55.0	57.9	58.4	434-460 bp	1.0	82.3
trnD-GUC-trnY-GUA_p2	GTGCTCTGACCGATTGAACT	GACATGTCTACGCTGGTTCA	50.0	50.0	57.9	57.6	409-435 bp	1.0	82.3
trnD-GUC-trnY-GUA_p3	GGATCCTAGTTCGGGACTGA	GGGGACGGACTGTAAATTCG	55.0	55.0	57.9	58.4	491-517 bp	1.0	82.2
trnD-GUC-trnY-GUA_p4	GGATCCTAGTTCGGGACTGA	GACATGTCTACGCTGGTTCA	55.0	50.0	57.9	57.6	466-492 bp	1.0	82.2
trnD-GUC-trnY-GUA_p5	GGATCCTAGTTCGGGACTGA	GGGACGGACTGTAAATTCGT	55.0	50.0	57.9	57.6	490-516 bp	1.0	82.0