Hotspot

atpF-atpH

LSC · 13865-14359 · 495 bp

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Pi 0.0264

MarkerSeek score 72.00

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 495
variable_sites: 13
pi: 0.0264
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	616-618 bp	1.0	80.7
atpF-atpH_p2	CAGTGGCCTAAGGAAACGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.8	58.0	588-590 bp	1.0	80.3
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.9	58.3	603-605 bp	1.0	78.9
atpF-atpH_p4	CAGTGGCCTAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.8	58.3	575-577 bp	1.0	78.5
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CGTGGCATTAGCGCTTTTAT	50.0	45.0	57.9	57.2	602-604 bp	1.0	75.0