Hotspot

trnS-GCU-trnG-GCC

LSC · 8428-9711 · 1284 bp

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Pi 0.0419
MarkerSeek score 73.60
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1284
variable_sites
44
pi
0.0419
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.82
markerseek_score
73.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CTCGTCCAACGGATTAGCAA CGAACGAATCACACTTTTACCA 50.0 40.9 58.0 57.3 1229-1373 bp 1.0 64.9
trnS-GCU-trnG-GCC_p2 AACTCGTCCAACGGATTAGC CGAACGAATCACACTTTTACCA 50.0 40.9 58.0 57.3 1231-1375 bp 1.0 64.9
trnS-GCU-trnG-GCC_p3 ATTAGCAATCCGCCGCTTTA CGAACGAATCACACTTTTACCA 45.0 40.9 58.0 57.3 1217-1361 bp 1.0 64.7
trnS-GCU-trnG-GCC_p4 CTCGTCCAACGGATTAGCAA TCGAACGAATCACACTTTTACC 50.0 40.9 58.0 57.0 1230-1374 bp 1.0 62.8
trnS-GCU-trnG-GCC_p5 AACTCGTCCAACGGATTAGC TCGAACGAATCACACTTTTACC 50.0 40.9 58.0 57.0 1232-1376 bp 1.0 62.8