Hotspot

ycf1

SSC · 125134-130593 · 5460 bp

Back to Leucocoryne

Pi 0.00238

MarkerSeek score 53.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5460
variable_sites: 13
pi: 0.00238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGACCATCGAGAAACCCTTT	AACTTCAACAGCAGACTCCA	45.0	45.0	56.8	57.0	319 bp	1.0	62.7
ycf1_p2	TGACCATCGAGAAACCCTTT	ACTTCAACAGCAGACTCCAT	45.0	45.0	56.8	56.8	318 bp	1.0	60.9
ycf1_p3	ATGACCATCGAGAAACCCTT	AACTTCAACAGCAGACTCCA	45.0	45.0	56.5	57.0	320 bp	1.0	60.9
ycf1_p4	TGACCATCGAGAAACCCTTTT	AACTTCAACAGCAGACTCCA	42.9	45.0	57.5	57.0	319 bp	1.0	60.7
ycf1_p5	TGTATGACCATCGAGAAACCC	AACTTCAACAGCAGACTCCA	47.6	45.0	57.5	57.0	323 bp	1.0	60.4