Hotspot

psbA

LSC · 78-1139 · 1062 bp

Back to Leucocoryne

Pi 0

MarkerSeek score 29.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	TTGGGTACGACTCCTTCAAC	GTGCTTGGGAGTCCTTGAAA	50.0	50.0	57.2	58.0	1152 bp	1.0	73.2
psbA_p2	TTGGGTACGACTCCTTCAAC	AATACGTGTGCTTGGGAGTC	50.0	50.0	57.2	57.9	1159 bp	1.0	72.6
psbA_p3	GGTACGACTCCTTCAACGAT	GTGCTTGGGAGTCCTTGAAA	50.0	50.0	57.1	58.0	1149 bp	1.0	72.4
psbA_p4	GGTACGACTCCTTCAACGAT	AATACGTGTGCTTGGGAGTC	50.0	50.0	57.1	57.9	1156 bp	1.0	71.8
psbA_p5	GGGTACGACTCCTTCAACGA	GTGCTTGGGAGTCCTTGAAA	55.0	50.0	59.1	58.0	1150 bp	1.0	70.9