Hotspot

atpF

LSC · 11891-13281 · 1391 bp

Back to Leucocoryne

Pi 0.00288

MarkerSeek score 60.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1391
variable_sites: 4
pi: 0.00288
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TCTTTTTCGGAAACAAAGGGA	AAAAAGGGCGAGTCAAGTGA	38.1	45.0	55.9	57.7	1524-1531 bp	1.0	52.8
atpF_p2	TCTTTTTCGGAAACAAAGGGA	GGGCGAGTCAAGTGATACAA	38.1	50.0	55.9	57.6	1519-1526 bp	1.0	52.5
atpF_p3	ACTAATTTCGTCAGCTCGAA	AAAAAGGGCGAGTCAAGTGA	40.0	45.0	54.9	57.7	1568-1575 bp	1.0	52.3
atpF_p4	ACTAATTTCGTCAGCTCGAA	GGGCGAGTCAAGTGATACAA	40.0	50.0	54.9	57.6	1563-1570 bp	1.0	52.0
atpF_p5	TCTTTTTCGGAAACAAAGGGA	AGGGCGAGTCAAGTGATACA	38.1	50.0	55.9	58.4	1520-1527 bp	1.0	52.0