Hotspot

rpl32-trnL-UAG

SSC · 114487-115349 · 863 bp

Back to Leucocoryne

Pi 0.00348

MarkerSeek score 60.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 863
variable_sites: 3
pi: 0.00348
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGTGCGACAAACAAGTAAGA	TCCTAAGAGCAGCGTGTCTA	40.0	50.0	55.6	57.9	936-942 bp	1.0	59.7
rpl32-trnL-UAG_p2	TGTGCGACAAACAAGTAAGA	AGCAGCGTGTCTACCAATTT	40.0	45.0	55.6	57.8	929-935 bp	1.0	59.3
rpl32-trnL-UAG_p3	TGTGCGACAAACAAGTAAGA	CCTAAGAGCAGCGTGTCTAC	40.0	55.0	55.6	57.8	935-941 bp	1.0	59.2
rpl32-trnL-UAG_p4	TGTGCGACAAACAAGTAAGA	CTAAGAGCAGCGTGTCTACC	40.0	55.0	55.6	57.8	934-940 bp	1.0	59.2
rpl32-trnL-UAG_p5	TGTGCGACAAACAAGTAAGA	GAGCAGCGTGTCTACCAATT	40.0	50.0	55.6	58.3	930-936 bp	1.0	58.6