Hotspot

rbcL-accD

LSC · 57456-58164 · 709 bp

Back to Leucocoryne

Pi 0.0113

MarkerSeek score 65.20

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 709
variable_sites: 8
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	TTGCTGCTGCTTGTGAAGTA	CAACATCGAATTGAACCGCC	45.0	50.0	58.0	58.1	816-817 bp	1.0	79.7
rbcL-accD_p2	TTGCTGCTGCTTGTGAAGTA	ATCGAATTGAACCGCCACTT	45.0	45.0	58.0	58.2	812-813 bp	1.0	79.1
rbcL-accD_p3	TGCTGCTGCTTGTGAAGTAT	CAACATCGAATTGAACCGCC	45.0	50.0	57.8	58.1	815-816 bp	1.0	78.4
rbcL-accD_p4	TGCTGCTGCTTGTGAAGTAT	ATCGAATTGAACCGCCACTT	45.0	45.0	57.8	58.2	811-812 bp	1.0	77.8
rbcL-accD_p5	TTGCTGCTGCTTGTGAAGTA	TCGAATTGAACCGCCACTTT	45.0	45.0	58.0	58.4	811-812 bp	1.0	77.3