Pi
0.00341
MarkerSeek score
61.40
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AGAGAAAAAGCCGGCTATCG | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 986-994 bp | 1.0 | 80.2 |
| trnT-UGU-trnL-UAA_p2 | CGATAGAGAAAAAGCCGGCT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 990-998 bp | 1.0 | 80.2 |
| trnT-UGU-trnL-UAA_p3 | TCGATAGAGAAAAAGCCGGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.1 | 58.0 | 991-999 bp | 1.0 | 80.2 |
| trnT-UGU-trnL-UAA_p4 | AGAGAAAAAGCCGGCTATCG | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 994-1002 bp | 1.0 | 79.7 |
| trnT-UGU-trnL-UAA_p5 | CGATAGAGAAAAAGCCGGCT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 58.1 | 58.1 | 998-1006 bp | 1.0 | 79.7 |